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    10x Protocols: Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling -- University of Minnesota TMCs (CG000553 Rev A)

    IOx Genomics, Laura Niedernhofer

    Published: 2023-06-26 DOI: 10.17504/protocols.io.q26g7y2pkgwz/v1
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    Abstract

    10x Genomics protocol where you fix the whole tissue and then dissociate it in preparation for Chromium Single Cell Expression flex

    Protocol ID# (CG) and Revision letter provided here :

    10x protocol CG000553, Revision A-- Fixation and dissociation of whole tissue; preparation for 10x Chromium Flex scRNAseq
    

    Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.

    Steps

    1.

    10x Tissue Fixation+Dissociation, Rev A (Protocol CG000553).pdf

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