A streamlined solution for processing, elucidating and quality control of cyclobutane pyrimidine dimer sequencing data

Quanhu Sheng, Hui Yu, Mingrui Duan, Scott Ness, Jiapeng He, Huining Kang, Limin Jiang, John J. Wyrick, Peng Mao, Yan Guo

Published: 2021-03-16 DOI: 10.1038/s41596-021-00496-3

cyclobutane pyrimidine dimers

UV-induced DNA damage

CPD-Seq

data processing protocol

genome-wide analysis

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UV radiation may lead to melanoma and nonmelanoma skin cancers by causing helix-distorting DNA damage such as cyclobutane pyrimidine dimers (CPDs). These DNA lesions, if located in important genes and not repaired promptly, are mutagenic and may eventuall
Introduction
- CPD sequencing
- Overview of the protocol
Materials
Procedure
Troubleshooting
- Steps 8, 12, 14, 15 and 17
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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A streamlined solution for processing, elucidating and quality control of cyclobutane pyrimidine dimer sequencing data

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