An in vitro batch fermentation protocol for studying the contribution of food to gut microbiota composition and functionality

Sergio Pérez-Burillo, Silvia Molino, Beatriz Navajas-Porras, Álvaro Jesús Valverde-Moya, Daniel Hinojosa-Nogueira, Alicia López-Maldonado, Silvia Pastoriza, José Ángel Rufián-Henares

Published: 2021-06-03 DOI: 10.1038/s41596-021-00537-x

in vitro fermentation

gut microbiota

food functionality

batch fermentation

personalized nutrition

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Knowledge of the effect of foods on gut microbiota composition and functionality is expanding. To isolate the effect of single foods and/or single nutrients (i.e., fiber, polyphenols), this protocol describes an invitro batch fermentation procedure to b
Introduction
Materials
Procedure
- In vitro fermentation
Troubleshooting
Timing (estimated for 50 fermentation tubes)
Anticipated results
References
ACKNOWLEDGMENTS

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An in vitro batch fermentation protocol for studying the contribution of food to gut microbiota composition and functionality

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